%0 Journal Article
%A Wenhui GUO
%A Yue WANG
%A Lin WU
%A Jianfei YANG
%A Ling YANG
%A Peng ZHANG
%A Haibo WU
%A Hailong SHEN
%T Effects of Exogenous Flavonoids on Embryogenic Callus Proliferation and Somatic Embryogenesis of Korean Pine
%D 2025
%R 10.7525/j.issn.1673-5102.2025.01.014
%J Bulletin of Botanical Research
%P 130-138
%V 45
%N 1
%X <p>Flavonoids play important roles in plant somatic embryogenesis. In order to explore the effect of flavonoids on somatic embryogenesis of Korean pine（<i>Pinus koraiensis</i>）， the embryogenic calluses were used as materials. The proliferation rate of embryogenic callus and the number of somatic embryos， and oxidative stress indexes in embryogenic callus were assessed after adding flavonoids（quercetin， naringenin， catechin） in different concentrations and phenylalanine aminolytic lyase inhibitor（2-aminoindan-2-phosphonate hydrochloride）. The results showed that the proliferation rate of embryogenic callus was significantly decreased with increasing levels of flavonoids， while the number of somatic embryos induced increased first then decreased， and the number of somatic embryos reached the peak under the treatment of exogenous flavonoids at 20 μmol<strong>?</strong>L<sup>-1</sup>. After treatment with flavonoids， the total reactive oxygen species（ROS） level， hydrogen peroxide（H<sub>2</sub>O<sub>2</sub>） content and malondialdehyde（MDA） content were reduced in embryogenic callus； the superoxide dismutase（SOD） activity was increased； the catalase（CAT）， peroxidase（POD）， ascorbate peroxidase（APX） and glutathione peroxidase（GPX） activities were decreased. In summary， exogenous flavonoids might regulate cell proliferation and callus differentiation by affecting the level of oxidative stress indexes in embryogenic callus， hence， promote the formation of somatic embryos of Korean pine.</p>
%U https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2025.01.014